From the lab of Jürgen Knoblich at the Institute of Molecular Biotechnology, Vienna, Science has published a CRISPR loss of function (LOF) screen in human cerebral organoids. By combining cell-barcoding with CRISPR-cell lineage tracing the authors were able to distinguish loss of proliferation following gRNA-mediated gene disruption from cell growth variability in 3D culture systems.
Reference: Esk C., Lindenhofer D., Haendeler S., Wester R.A., Pflug F., Schroeder B., Bagley J.A., Elling U., Zuber J., von Haeseler A., Knoblich J.A. (2020) A human tissue screen identifies a regulator of ER secretion as a brain size determinant. Science. DOI: 10.1126/science.abb5390.
Keywords: CRISPR LOF screen, Barcodes, Lineage Tracing, 3D Culture