New Tools: Loss of function screen in organoids using CRISPR-LIGHT, Lineage Tracing at Cellular resolution in Heterogenous Tissue

From the lab of Jürgen Knoblich at the Institute of Molecular Biotechnology, Vienna, Science has published a CRISPR loss of function (LOF) screen in human cerebral organoids. By combining cell-barcoding with CRISPR-cell lineage tracing the authors were able to distinguish loss of proliferation following gRNA-mediated gene disruption from cell growth variability in 3D culture systems.

Reference: Esk C., Lindenhofer D., Haendeler S., Wester R.A., Pflug F., Schroeder B., Bagley J.A., Elling U., Zuber J., von Haeseler A., Knoblich J.A. (2020) A human tissue screen identifies a regulator of ER secretion as a brain size determinant. Science. DOI: 10.1126/science.abb5390.

Keywords: CRISPR LOF screen, Barcodes, Lineage Tracing, 3D Culture

New Tools: CRISPRoff, light-degradable sgRNAs

Carlson-Stevermer et al. incorporate photocleavable residues in sgRNAs enabling inactivation upon exposure to UV light. CRISPRoff provides spatial and temporal control of gene editing activities.

Reference: Carlson-Stevermer, J., Kelso, R., Kadina, A. et al. (2020) CRISPRoff enables spatio-temporal control of CRISPR editing. Nat. Commun. 11, 5041.

Keywords: CRISPRoff, sgRNA cleavage, Spatio-Temporal control

Questions? Email:

World CRISPR Day 2020 (Virtual Conference)

Tuesday, October 20, 2020.

A virtual CRISPR conference “Accelerating Discovery to Clinic and Real-World Applications” will take place on Tuesday October 20. Among the speakers are Jennifer Doudna, Waseem Qasim, Jacob Corn, and Jonathan Gootenberg. For more information and free registration check:

Keywords: Virtual Conference, CRISPR research, CRISPR applications

Questions? Email: