Scientific advances: Genome-wide CRISPR screens in cancer cells:
March 25
Gilad, Y., et al. (2021) A genome-scale CRISPR Cas9 dropout screen identifies synthetically lethal targets in SRC-3 inhibited cancer cells. Comm. Bio. https://doi.org/10.1038/s42003-021-01929-1
Breast Cancer, MCF7, GeCKOv2 library, Steroid receptor coactivator 3 (SRC-3) inhibitor
March 24
Barkovskaya, A., et al. (2021) Detection of phenotype-specific therapeutic vulnerabilities in breast cells using a CRISPR loss-of-function screen. Mol. Oncol. https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.12951
Isogenic human breast epithelial cell lines D492 and D492M, druggable genome screen, fluorouracil (5-FY), epithelial – mesenchymal transition (MET)
March 23
Van der Weyden, L., et al. (2021) CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonization. Comm. Bio. https://doi.org/10.1038/s42003-021-01912-w
Melanoma, cell surface protein LRRN4CL, CRISPR activation, B16-F0 mouse melanoma cell line, in vivo screen
March 19
Ahmed, M., et al. (2021) CRISPRi screens reveal a DNA methylation mediated 3D genome dependent causal mechanism in prostate cancer. Nat. Comm. https://doi.org/10.1038/s41467-021-21867-0
Prostate Cancer, SNPs, CRISPR interference, sgRNA tiling, A549, 22Rv1, and V16A cells
March 19
Cheng, C., et al. (2021) CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radio-resistance by stabilizing β-catenin. Oncogene https://doi.org/10.1038/s41388-021-01692-x
Radiation resistance, plakophilin 2 (PKP2), NHEJ, A549 lung cancer cells, GeCKOv2 library
Keywords: CRISPR, screen, genome-wide, cancer, synthetic lethality
Tools. CRISPR/Cas gene editing in human induced pluripotent stem cells.
March 24
The journal Methods in Molecular Biology published three protocols involving gene editing in human induced pluripotent stem cells (hiPSCs).
Brandão, K.A., et al. (2021) CRISPR/Cas9-mediated introduction of specific heterozygous mutations in human induced pluripotent stem cells. In: Methods in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/7651_2021_368
Benetó N., et al. (2021) Genome editing using Cas9-gRNA ribonucleoprotein in human pluripotent stem cells for disease modeling. In: Methods in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/7651_2021_374
Sanjurjo-Soriano C., et al. (2021) CRISPR/Cas9-mediated genome editing to generate clonal iPSC Lines. In: Methods in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/7651_2021_362
Keywords: CRISPR, (h)iPSCs, protocols
Questions? Email: crispr@amsterdamumc.nl