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New Tools: a watchdog for translocations and off-target editing

CRISPector algorithm can spot potential off-target modifications and quantify translocations. Amit, I. et al. (2021) CRISPECTOR provides accurate estimation of
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New Tools: CRISPR C>G, G>C base editor

Chen, L. et al. (2021) Programmable genome editing with CRISPR-Cas9-directed base excision repair proteins Nature Communications.DOI: 10.1038/s41467-021-21559-9 Keywords: CRISPR, Base
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Scientific Advances: Pancreatic cancer: tracking the gene changes underlying metastasis

Researchers from the University of Pennsylvania have developed a new CRISPR-based method to help trace the lineage and gene expression
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Scientific Advances: Base editing in hematopoietic stem cells rescues sickle cell disease

In the lab of David Liu, a CRISPR base editor was successfully applied to correct mutations in the β-globin gene
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Scientific Advances: Lowering cholesterol, the CRISPR way

CRISPR base editors delivered via a single infusion of lipid nanoparticles to the liver efficiently reduced expression of the PCSK9
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At a glance

May 13. Prime editing efficiencies in primary human cells and ZebrafishFor more information, see Petri, K., et al. (2021) CRISPR
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We have established an online community platform for all CRISPR users of the Amsterdam UMC. Please register or sign in to ask questions about CRISPR technology and discuss your problems and ideas.
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We regularly organize courses for AUMC PhD students and PostDocs. Please register to participate.
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Tools and links

We have assembled a selection of useful CRISPR tools and links.
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