New Tools: Base Editing with Minimal Bystander Effects

Up till now the application of base editors was limited due to the fact that other cytosines in the proximity of the intended C->T alteration would also be converted. Now, Lee et al. describe an engineered high-fidelity base editor to overcome previous limitations.

Reference: Lee et al. Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects. Science Advances. (2020)  Vol. 6, no. 29, eaba1773. DOI: 10.1126/sciadv.aba1773

Keywords: base editor, APOBEC

Questions? Email: crispr@amsterdamumc.nl