Carlson-Stevermer et al. incorporate photocleavable residues in sgRNAs enabling inactivation upon exposure to UV light. CRISPRoff provides spatial and temporal control of gene editing activities.

Reference: Carlson-Stevermer, J., Kelso, R., Kadina, A. et al. (2020) CRISPRoff enables spatio-temporal control of CRISPR editing. Nat. Commun. 11, 5041. https://doi.org/10.1038/s41467-020-18853-3

Keywords: CRISPRoff, sgRNA cleavage, Spatio-Temporal control

Questions? Email: crispr@amsterdamumc.nl