Scientific advances / Screens / New Tools: Gene editing in difficult to manipulate dendritic cells

In the lab of Jonathan Weissman a non-viral electroporation-based method was developed to effectively create gene knockouts in primary monocyte-derived dendritic cell cultures. The approach relies on CRISPR/Cas9 ribonucleoprotein (RNP) complexes and a Nucleofector-4D (Lonza) for delivery. The authors reportedly achieved gene disruption efficiencies over 94% based on the targeting of more than 300 genes. In CRISPR screens, genetic mechanisms were identified by which dendritic cells mediate immune responses to foreign entities. This underlines the potential of this approach to systematically unravel cellular immune signaling pathways.

Jost, M., et al. (2021) CRISPR-based functional genomics in human dendritic cells. eLife 10:e65856.

Keywords: CRISPR screen, dendritic cells, electroporation, RNP, immune response

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