In recent years distinct variants have been developed based on the originally adapted nuclease Streptococcus pyogenes Cas9. In a recent issue of Nature Biotechnology, Kim et al. have analyzed the sequence-specific cleavage activity of several Cas9 variants and developed an online prediction tool to help you find the right Cas9 for your experiment.

Reference: Kim et al. Prediction of the sequence-specific cleavage activity of Cas9 variants. Nat Biotechnol (2020). https://doi.org/10.1038/s41587-020-0537-9

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